KMID : 1005220160310030207
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Journal of Embryo Transfer 2016 Volume.31 No. 3 p.207 ~ p.213
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Effect of glutathione on tetraploid embryo development in the pigs
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Kim Hwa-Young
Lee Sang-Hee Hwangbo Yong Lee Seung-Tae Lee Eun-Song Park Choon-Keun
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Abstract
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The objective of this study was to investigate to influence of glutathione (GSH) on development and antioxidant enzyme activity in tetraploid porcine embryos. Tetraploid embryos were produced using parthenogenetic 2-cell embryo by electrofusion method. Tetraploid embryo development was observed every 24 hours and intracellular antioxidant enzyme activity was measured at 120 hours after electrofusion. The 4-cell to 16-cell stage tetraploid embryos was increased in 100 and 500 ¥ìM GSH-treated groups compared control group at 48 hours (P < 0.05) but cleavage rates were not significantly different among the GSH treatment groups at 48, 72, 96, and 120 hours. Blastocyst formation was significantly increased by 300 and 500 ¥ìM GSH at 120 hours in tetraploid embryos (P < 0.05). But blastocyst cell number were not significantly different among the GSH treatment groups (16.4 ¡¾ 0.8, 16.8 ¡¾ 2.6, 18.5 ¡¾ 2.8 and 17.5 ¡¾ 1.8). The intracellular antioxidant enzyme level was increased in 500 ¥ìM GSH compared to 0 and 100 ¥ìM GSH (P < 0.05). We suggest that GSH may be improve development of tetraploid embryo in pigs.
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KEYWORD
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Tetraploid , embryo development , electrofusion , glutathione , pig
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